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Peripheral blood mononuclear cells isolated from healthy individuals using Ficoll and co-cultured with PHA for 8 h at 37 °C in humidified atmosphere containing 50 mL/L CO2. The expression of FasL on PBL before and after PHA stimulation was detected by special antibody using flow cytometer. Open bar indicates the percent of FasL on cells surface before PHA activation. Shadow bar shows the percent of FasL on cell surface after PHA stimulation.
