Table 2.
Plasma parameters and glucose turnover data in control and lipid-infusion rats during euglycemic hyperinsulinemic claming (mean ± SE)
| Group | Basal |
Clamping time (min) |
||||
| 120 | 200 | 220 | 230 | 240 | ||
| Glucose (mmol/L) | ||||||
| Control (n = 12) | 5.2 ± 0.1 | 5.3 ± 0.1 | 5.0 ± 0.2 | 5.2 ± 0.1 | 5.2 ± 0.2 | 5.4 ± 0.2 |
| Lipid (n = 12) | 5.1 ± 0.2 | 5.3 ± 0.2 | 5.2 ± 0.2 | 5.3 ± 0.2 | 5.5 ± 0.2 | 5.5 ± 0.2 |
| FFA (μmol/L) | ||||||
| Control (n = 12) | 672.5 ± 92.2 | 642.3 ± 104.8 | 240.6 ± 20.9 | 221.3 ± 27.4 | 201.8 ± 29.8 | 183.1 ± 21.4 |
| Lipid (n = 12) | 741.9 ± 50.6 | 2807.2 ± 348.8bd | 3086.9 ± 495.2bd | 2518.8 ± 416.7bd | 2241.2 ± 431.0bd | 2346.4 ± 238.5bd |
| Insulin (mU/L) | ||||||
| Control (n = 12) | 30.3 ± 2.4 | 34.7 ± 6.4 | 89.5 ± 7.9 | 90.7 ± 7.4 | 88.3 ± 5.9 | 101.3 ± 6.3 |
| Lipid (n = 12) | 27.9 ± 2.2 | 31.4 ± 3.3 | 84.2 ± 6.7 | 93.3 ± 8.9 | 103.5 ± 16.3 | 104.5 ± 14.8 |
| GIR (mg/kg· min) | ||||||
| Controls (n = 12) | 0 | 32.8 ± 1.7 | 34.4 ± 1.6 | 34.4 ± 1.6 | 34.7 ± 1.7 | |
| Lipid (n = 12) | 0 | 12.7 ± 1.3b | 12.9 ± 1.6b | 12.9 ± 1.8b | 12.0 ± 1.9b | |
| GRd (mg/kg·min) | ||||||
| Controls (n = 12) | 19.0 ± 4.5 | 43.1 ± 6.1d | 40.1 ± 6.7d | 35.5 ± 6.8d | 33.2 ± 3.2d | |
| Lipid (n = 12) | 18.7 ± 3.0 | 36.3 ± 3.1d | 38.3 ± 4.4d | 32.4 ± 4.5d | 35.1 ± 3.9d | |
| HGP (mg/kg·min) | ||||||
| Controls (n = 12) | 19.0 ± 4.5 | 14.2 ± 4.9c | 10.2 ± 4.4d | 3.1 ± 1.9d | 2.3 ± 0.9d | |
| Lipid (n = 12) | 18.7 ± 3.0 | 23.4 ± 4.3bc | 25.3 ± 3.7bd | 21.5 ± 3.5b | 23.2 ± 3.1bd | |
FFA: Free fatty acids. GIR: Glucose infusion rate. GRd: Glucose disappearance rate. HGP: Hepatic glucose production. aP < 0.05;
b
P < 0.01 vs control;
c
P < 0.05;
d
P < 0.01 vs Basal. The basal values of HGP were determined at 120 min.