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. 2007 Dec 28;13(48):6538–6548. doi: 10.3748/wjg.v13.i48.6538

Figure 4.

Figure 4

Light micrographs of tissue sections from murine liver (after 30 d of tumour transplantation) showing immunostaining of metallothionein (MT) with anti-rat MT-1 antibody and 3, 3'-diaminobenzidine tetrahydrochloride (DAB). A and B: Strong immunostaining of MT protein in EAC Control mice; C: Reduced immunostaining of MT in EAC + ALE-treatment mice. ALE: Aqueous leaf extract of A. ilicifolius; EAC: Ehrlich ascites carcinoma. Arrow Head ( ) indicates intense immunostaining of MT protein with prominent focal expression and isolated clusters of MT-positive cells. Arrow (↑) indicates scattered/individual MT immunopositive cells. Magnification, A: × 100; B and C: × 270.