Table 1.
Exon | Sequence of the primer | Product of PCR (bp) | Temperature for annealing (°C) |
1S | 5’TCTGCGGAGAGGGGGAGAGCAG3’ | 280 | 58 |
1A | 5’GCGCTACCTGATTCCAATTC3’ | ||
2S | 5’TTCCTTTCCGTCATGCCGG 3’ | 394 | 56 |
2A | 5’GTACAAATTCTCAGATCATCAGTCCTC3’ | ||
3S | 5’GGATGTTCCACACATCTTTG3’ | 189 | 52 |
3A | 5’ATGAAAACTACGAAAGCGGG3’ |
S: Sense; A: Antisense.