Figure 4.
Bradyrhizobium sp. LTSP M299 contains genes enriched in the mineral soil layer, typically occurring in clusters on genomic islands or putative extrachromosomal elements. (a) The abundance of LTSP M299 ORFs in metagenomes from mineral and organic soil layers shows that most of the ORFs enriched in the mineral layer (FDR-q value ⩽0.01) are not shared with the closely related strain LTSP 885 isolated from the organic layer. (b) Gaps between RBHs indicate genomic regions present in the LTSP M299 genome but absent from LTSP 885, and vertical red lines indicate ends of contigs. Most of the ORFs enriched in the mineral layer (103 of 133) occur in genomic regions containing 10 or more ORFs not shared with LTSP 885, often in discrete clusters within these regions. Percent GC, frequency of tetra-A homopolymer regions provide supporting evidence that the non-shared genomic regions were acquired by horizontal transfer.