Figure 6.

(a, b) Number of protein-coding genes shared between ‘Ca. Methylopumilus planktonicus' MMS-2-53 (a) or ‘Ca. Methylopumilus turicensis' MMS-10A-171 (b) and genome sequenced members of Methylophilaceae and a typical freshwater microbe (Polynucleobacter necessarius subsp. asymbioticus QLW-P1DMWA-1). Protein identity cutoffs of ⩾30% and ⩾70% (minLrap⩾0.8) were applied. (c, d) Reconstruction of methylotrophic pathways of ‘Ca. Methylopumilus planktonicus' MMS-2-53 (c) and ‘Ca. Methylopumilus turicensis' MMS-10A-171 (d). Genes involved in methylotrophy are given in gray (see Supplementary Table S2 for more information). Dashed lines indicate spontaneous reactions, gray lines indicate possible pathways for CH₃X oxidation. unkn. MT, unknown methyltransferase; CH₃OH, methanol; CH₃X, other C1 compounds, for example, methylamine; CH₂O, formaldehyde; HCOOH, formate; H₄F, tetrahydrofolate; H₄MPT, tetrahydromethanopterin; H6P; hexulose-6-phosphate; F6P, fructose-6-phosphate; G6P, glucose-6-phosphate; 6PGL, 6-phosphogluconolactone; 6PG, 6-phospho-gluconate; Ru5P, ribulose-5-phosphate; RuMP cycle, ribulose monophosphate cycle.