Table 1. Differences in gene family profiles of conventional and AA classes attributable to soil layer and harvesting treatments.

	Conventional classes		Auxiliary activities
	F or t	R2	F or t	R2
All samples
Layer	21.982*	0.76	22.51*	0.75
Harvesting treatment	6.87***	0.17	5.54***	0.17
Residuals		0.07		0.08
Mineral layer
Harvesting treatment	3.63**	0.58	1.24	NS
OM0 vs OM1	2.26*
OM0 vs OM2	2.86*
OM0 vs OM3	2.02*
OM1 vs OM2	2.23*
OM1 vs OM3	1.25
OM2 vs OM3	0.91
Organic layer
Harvesting treatment	11.85**	0.80	10.98**	0.79
OM0 vs OM1	4.82*		4.92**
OM0 vs OM2	2.22*		2.21*
OM1 vs OM2	3.09*		2.50*

Abbreviations: AA, auxiliary activity; NS, not significant; OM, organic matter.

Permutational multivariate analysis of variance (PERMANOVA) based on Bray–Curtis distances. Multivariate dispersion was significantly different (P<0.05) when analyzing all samples together but nonsignificant when each soil layer was analyzed separately. Global test results yielded pseudo-F values, whereas pairwise comparisons yielded t-values (italicized). P-values are based on permutation for the general tests, and on Monte Carlo permutation for pairwise tests. *P<0.05; **P<0.01; ***P<0.001.