Table 2. Results of 454 pyrosequencing of bacterial and fungal communities across all samples (177 in total).
| Variable | Raw |
Rarefied |
|||
|---|---|---|---|---|---|
| Total | Ambient | Drought | Nitrogen | ||
| Bacteria | |||||
| Total sequences | 1 197 853 | 110 271 | 36 134 | 35 511 | 38 626 |
| Median sequences/sample | 5953 | 623 | 623 | 623 | 623 |
| Total OTUs (97% similarity) | 2199 | 935 | 573 | 621 | 589 |
| Singleton OTUs | 35% | 38% | 36% | 36% | 37% |
| Relative abundance of top 20 OTUs | 73% | 74% | 74% | 75% | 73% |
| Median OTUs/sample | 211 | 80 | 84 | 77 | 85 |
| Mean Shannon diversity (±s.e.) | — | 4.46 (0.07) | 4.49 (0.13) | 4.35 (0.13) | 4.54 (0.13) |
| Fungi | |||||
| Total sequences | 1 261 095 | 401 613 | 136 140 | 129 333 | 136 140 |
| Median sequences/sample | 6507 | 2269 | 2269 | 2269 | 2269 |
| Total OTUs (97% similarity) | 830 | 602 | 455 | 412 | 420 |
| Singleton OTUs | 30% | 25% | 24% | 29% | 27% |
| Relative abundance of top 20 OTUs | 94% | 93% | 92% | 94% | 93% |
| Median OTUs/sample | 97 | 64 | 71.5 | 59 | 65.5 |
| Mean Shannon diversity (±s.e.) | — | 2.54 (0.04) | 2.75 (0.09) | 2.37 (0.06) | 2.49 (0.07) |
Abbreviation: OTU, operational taxonomic unit.