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The system of PCR
| Constituents | Volume/per tube (μL) | Final concentration |
| Water | 36 | |
| 10×PCR buffer | 5 | 1× |
| 4dNTP, 2.5mmol/L /each | 4 | 0.2mmol/L each |
| primer 1.25 μmol/L | 1 | 0.5 μmol/L |
| primer 2.25 μmol/L | 1 | 0.5 μmol/L |
| MgCl2, 50 mmol/L | 1.5 | 1.5 mmol/L |
| Taq DNA polymerase, 5MU/L | 0.5 | 0.1 MU/L |
| Template DNA | 1 |
