Fig. 2.
2W:I-Ab–specific T cells differentiate into Th17 cells in the CLNs after i.n. Sp-2W inoculation. Plots represent 2W:I-Ab–specific T cells in 2W:I-Ab tetramer-enriched samples from the indicated organs and at the indicated times after i.n. infection with Sp-2W bacteria. For NALT and CLNs, three mice were pooled per sample; for spleen, one mouse was used per sample. Numbers over each gate indicate the total number of 2W:I-Ab–specific T cells in that organ. Numbers in the lower right corner of each gate indicate the percentage of 2W:I-Ab–specific T cells in the sample. Data are representative of three independent experiments.