Fig. S4.

Assessment of calreticulin expression in cells. CRT^−/− MEFs were infected with retroviruses to express the indicated calreticulin constructs. Following infection, cells were lysed and calreticulin levels in the cell lysates were assessed by immunoblotting, followed by detection via chemiluminescence (Left) or fluorescence (Right). The figure shows representative immunoblots from 12 to 15 [mCRT(WT) and Vec], six to nine [mCRT(K7A) and mCRT(D12A)], five [mCRT(R19A)], or two to three [mCRT(K63A) and mCRT(D311A)] independent infections.