Fig. 7.

Gut migDCs from Limited mice lost the steady-state migDC signature. (A) Gene expression profile of FACS-sorted CD103⁺ DCs and CD103⁻ DCs from the MLN of Cα^+/o or Limited mice. A dot plot shows the comparison between fold-change (FC) of CD103⁺ DCs/CD103⁻ DCs of Limited mice vs. that of Cα^+/o mice (Left). Top 50 genes of the signature genes set of steady-state migDCs (22) is highlighted in green (Table S1). A heatmap shows the relative expression of migDC signature genes among CD103⁻ DCs and CD103⁺ DCs from Cα^+/o mice (C) or Limited mice (L) (Right). (B) Quantative RT-PCR analysis for Aldh1a2 of sorted CD103⁺ DCs or CD103⁻ DCs from MLN of Cα^+/o or Limited mice (n = 3 per each group).