Fig. 3.

Actin morphology and flux for D. discoideum depend on details of the nano/microtopography. (A–L) Eight-micron sawteeth (A–F) and 2-μm sawteeth (G–L). (A and G) Top-view confocal micrographs of F-actin on sawteeth. (B, C, H, and I) Confocal micrographs of cross-sections perpendicular to the ridges going through the sawtooth minima (B and H) and maxima (C and I). (D and J) Actin-wave directionality. (E and K) Average actin flux around a sawtooth. (F and L) Sixty-frame (2.55-min) space/time plot of actin waves along a ridge. (Scale bars: A–C and G and I, 3 μm; F, 16 μm; L, 4 μm.)