Table S1.
Primer | Sequence, 5′-3′ |
twd40-2-3 genotyping | |
RP | TGTGCTTTCCCTATCACCATC |
LP | TAGGGGACTATGACATGCTCG |
BP LBb1.3 | ATTTTGCCGATTTCGGAAC |
GFP-TWD40-2 cloning | |
SacI prom F | CGAGCTCTGTACATGGATTGTCAGGC |
SpeI prom R | ACTAGTCTTCGATCTCTCTCTCTCTCTC |
TWD40-2 F | ATGTTGCGGGCGAGAGCATTTCG |
TWD40-2 R | TTAGCCATTCAAGAAATCAAAATCTATTG |
Qualitative RT-PCR | |
P1 F | ATGTTGCGGGCGAGAGCATTTCG |
P1 R | CTTGCTTTGGCACATCACGACC |
P2 F | GGTGGGGTGGTGGCTGTTG |
P2 R | CTCACAGAAAGAACTGAGTATG |
ACT2 F | ATGGCTGAGGCTGATGATATTCAACC |
ACT2R | CTCTCTGTAAGGATCTTCATGAGG |
AP2M F | CCTGTGCGTCAGATTGGTGGCTGCTCC |
AP2M R | GAATCTGGTCAGGTTCACACACTGGTG |
Real-time qPCR | |
ACT2 F | TGGTCGTACAACCGGTATTGTG |
ACT2 R | GCTGTTGTGGTGAACATGTAACC |
AP2M F | TTGTTGGATGAGATTATGGACTTTG |
AP2M R | CCAACAGCACCTGTAACTTGTAATGT |
TWD40-2 UP F | TCCCCTGCTCCATCGACTAA |
TWD40-2 UP R | CGAAAGAAACTCCATGCAGAGA |
TWD40-2 DN F | TGCAGCTTCATCGGCTAGTG |
TWD40-2 DN R | CTTCGGGATGTGCGATAACC |
BP, T-DNA border primer; F, forward; LP, left genomic primer; R, reverse; RP, right genomic primer.