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. 2015 Sep 28;112(41):12870–12875. doi: 10.1073/pnas.1509292112

Table S1.

Sequences of the primers used in this study

Primer Sequence, 5′-3′
twd40-2-3 genotyping
 RP TGTGCTTTCCCTATCACCATC
 LP TAGGGGACTATGACATGCTCG
 BP LBb1.3 ATTTTGCCGATTTCGGAAC
GFP-TWD40-2 cloning
 SacI prom F CGAGCTCTGTACATGGATTGTCAGGC
 SpeI prom R ACTAGTCTTCGATCTCTCTCTCTCTCTC
 TWD40-2 F ATGTTGCGGGCGAGAGCATTTCG
 TWD40-2 R TTAGCCATTCAAGAAATCAAAATCTATTG
Qualitative RT-PCR
 P1 F ATGTTGCGGGCGAGAGCATTTCG
 P1 R CTTGCTTTGGCACATCACGACC
 P2 F GGTGGGGTGGTGGCTGTTG
 P2 R CTCACAGAAAGAACTGAGTATG
 ACT2 F ATGGCTGAGGCTGATGATATTCAACC
 ACT2R CTCTCTGTAAGGATCTTCATGAGG
 AP2M F CCTGTGCGTCAGATTGGTGGCTGCTCC
 AP2M R GAATCTGGTCAGGTTCACACACTGGTG
Real-time qPCR
 ACT2 F TGGTCGTACAACCGGTATTGTG
 ACT2 R GCTGTTGTGGTGAACATGTAACC
 AP2M F TTGTTGGATGAGATTATGGACTTTG
 AP2M R CCAACAGCACCTGTAACTTGTAATGT
 TWD40-2 UP F TCCCCTGCTCCATCGACTAA
 TWD40-2 UP R CGAAAGAAACTCCATGCAGAGA
 TWD40-2 DN F TGCAGCTTCATCGGCTAGTG
 TWD40-2 DN R CTTCGGGATGTGCGATAACC

BP, T-DNA border primer; F, forward; LP, left genomic primer; R, reverse; RP, right genomic primer.