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. 2015 Sep 28;112(41):12675–12680. doi: 10.1073/pnas.1509090112

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Fig. S1. — Comparison of plant-produced PG9 and RSH with ^CHOPG9. Coomassie Brilliant Blue staining of purified PG9 and RSH after separation by SDS/PAGE under (Upper) reducing and (Lower) nonreducing conditions. Heavy chain (HC) bands are indicated by a black arrowhead. A black diamond indicates the light chain (LC) band of ^CHOPG9, which is N-glycosylated and therefore, has a higher molecular mass than the LC of the plant-produced mAbs (white arrowhead). Arrows indicate fully assembled IgG molecules separated under nonreducing conditions. Lane 1, ^ΔXFPG9; lane 2, ^ΔXFPG9_Sulf; lane 3, ^ΔXFPG9_SulfSia; lane 4, ^ΔXFRSH; lane 5, ^ΔXFRSH_Sulf; lane 6, ^ΔXFRSH_SulfSia; lane 7, ^CHOPG9.