. 2015 Sep 28;112(41):12675–12680. doi: 10.1073/pnas.1509090112

Table S1.

Tyrosine sulfation of PG9 and RSH

mAb	TPST1 construct	OD₆₀₀	0S (%)	1S (%)	2S (%)
^ΔXFPG9_Sulf	p^FullhsTPST1	0.01	91.5 ± 7.0	8.2 ± 6.4	0.3 ± 0.7
^ΔXFPG9_Sulf	p^FullhsTPST1	0.05	83.4 ± 13.9	16 ± 13.1	0.6 ± 0.9
^ΔXFPG9_Sulf	p^FullhsTPST1	0.2	83.7 ± 8.8	15.6 ± 8.2	0.7 ± 0.9
^ΔXFPG9_Sulf	p^FullhsTPST1	0.8	82.8 ± 11.7	16.8 ± 11	0.4 ± 0.7
^ΔXFPG9_Sulf	p^Fut11hsTPST1	0.01	73.7 ± 5.8	17.8 ± 3.3	8.6 ± 2.6
^ΔXFPG9_Sulf	p^Fut11hsTPST1	0.05	57.4 ± 0.8	32.1 ± 2.3	10.6 ± 3.1
^ΔXFPG9_Sulf	p^Fut11hsTPST1	0.2	59.6 ± 5.8	28.1 ± 5.4	12.3 ± 5.4
^ΔXFPG9_Sulf	p^Fut11hsTPST1	0.8	58.7 ± 8.7	32.7 ± 10.8	8.6 ± 2.1
^ΔXFPG9_Sulf	p^RSThsTPST1	0.01	69.5 ± 8.9	20.6 ± 6.9	9.9 ± 2.3
^ΔXFPG9_Sulf	p^RSThsTPST1	0.05	53.9 ± 6.1	30.8 ± 2.3	15.3 ± 3.8
^ΔXFPG9_Sulf	p^RSThsTPST1	0.2	57.2 ± 10.4	29.3 ± 7.4	13.5 ± 5.1
^ΔXFPG9_Sulf	p^RSThsTPST1	0.8	53.7 ± 11.1	36.8 ± 11.8	9.5 ± 2.7
^ΔXFRSH_Sulf	p^RSThsTPST1	0.2	57.4 ± 7.6	29.6 ± 5.2	13.1 ± 3.6
^CHOPG9			18.5 ± 1.5	60.4 ± 3.9	21.1 ± 5.4

graphic file with name pnas.1509090112sfx01.jpg

Relative amounts of unsulfated (0S), monosulfated (1S), and disulfated (2S) PG9 and RSH when coexpressed with different hsTPST1 constructs in plants. The sulfation status of ^CHOPG9 is shown for comparison. Data are presented as means ± SD of two to nine analyses.