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. 2015 Sep 28;112(41):12675–12680. doi: 10.1073/pnas.1509090112

Table S2.

N-glycosylation of PG9 and RSH expressed in plants and CHO cells

Glycan ΔXFPG9 (%) ΔXFPG9Sulf (%) ΔXFPG9SulfSia (%) ΔXFRSH (%) ΔXFRSHSulf (%) ΔXFRSHSulfSia (%) CHOPG9 (%)
UGnF* 0.0 0.0 0.0 0.0 0.0 0.0 1.5
MGn* 1.8 2.1 1.3 1.5 2.1 1.3 0.5
MGnF* 0.0 0.0 0.0 0.0 0.0 0.0 3.8
GnGn* 45.0 50.9 17.5 41.1 56.1 18.1 3.0
GnGnX* 1.3 1.9 0.5 0.8 1.0 0.2 0.0
GnGnF* 0.8 1.4 0.1 0.5 0.5 0.2 69.8
MA* 0.1 0.2 0.9 0.1 0.0 1.4 0.0
GnA* 0.6 0.3 9.8 1.2 1.1 12.3 0.3
GnAF* 0.1 0.1 0.2 0.2 0.0 0.5 17.7
AA* 0.0 0.1 17.6 0.0 0.1 24.4 0.0
AAF* 0.0 0.0 1.1 0.0 0.0 0.7 2.5
MNa* 0.0 0.0 1.0 0.0 0.0 1.9 0.0
GnNa* 0.0 0.0 0.3 0.0 0.0 0.3 0.0
ANa* 0.0 0.0 2.9 0.0 0.0 6.4 0.0
ANaF* 0.0 0.0 0.6 0.0 0.0 0.7 0.3
NaNa* 0.0 0.0 0.3 0.0 0.0 0.6 0.0
NaNaF* 0.0 0.0 1.2 0.0 0.0 2.7 0.0
Man5 1.0 0.2 0.9 0.5 0.3 0.2 0.5
Man6 1.8 1.3 1.7 1.6 1.4 1.0 0.0
Man7 2.8 1.4 2.8 2.2 2.2 2.7 0.1
Man8 18.7 8.8 14.0 12.9 13.1 11.5 0.0
Man9 26.0 31.4 25.6 37.3 22.0 13.0 0.0
Gn 48.9 56.2 19.4 44.0 59.8 19.9 78.5
Gal 0.8 0.7 29.6 1.5 1.2 39.2 20.6
Sia 0.0 0.0 6.1 0.0 0.0 12.5 0.3
Man5-9 50.3 43.1 44.9 54.5 39.0 28.4 0.6
Fuc§ 0.9 1.5 3.2 0.7 0.5 4.8 95.6
*

N-glycan acronyms are based on the ProGlycAn nomenclature (www.proglycan.com).

Man5-9, oligomannosidic N-glycans with five to nine mannose residues.

Gn/Gal/Sia, sum of complex N-glycans with terminal GlcNAc/galactose/sialic acid residues; Man5-9, sum of oligomannosidic N-glycans with five to nine mannose residues.

§

Fuc, sum of core-fucosylated N-glycans.