Fig. S4.

MIND and Dam1c have different effects on the diffusion of Ndc80c. Mean squared displacement of indicated complexes plotted vs. time. Dotted lines show weighted linear fits used to calculate average diffusion constants, with error bars denoting SD. Diffusion constants for each complex are as follows: (blue) Ndc80c-GFP = 0.0343 ± 0.0012 μm²⋅s⁻¹, (red) Ndc80c-GFP + Dam1c = 0.0069 ± 0.0002 μm²⋅s⁻¹, (black) MIND-GFP/Ndc80c = 0.0141 ± 0.0004 μm²⋅s⁻¹, (green) MIND-GFP/Ndc80c + Dam1c = 0.0053 ± 0.0002 μm²⋅s⁻¹.