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Gel filtration profiles of MINDc-GFP/Ndc80c and MINDc-GFP/Ndc80-121c. Representative Sephacryl 400 gel filtration elution profiles for MINDc-GFP/Ndc80c co-complex (blue) and MINDc-GFP/Ndc80-121c (red). The gray-shaded bar indicates peaks that samples were taken from for experiments. Note that MIND-GFP/Ndc80c and MIND-GFP/Ndc80-121c elute at the same volume, indicating that the mutations in Ndc80-121c do not influence formation of higher order structures.
