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Purification of Bs-F(ab)2 after click chemistry conjugation of TRC105-Fab and CET-Fab. (A) Size exclusion chromatogram of the reaction mixture; the peak around 53 min corresponds to Bs-F(ab)2. (B) Chromatogram of the purified Bs-F(ab)2 and the starting materials. (C) Table showing the Bs-F(ab)2 generation yield. Yields were determined as the ratio between the two peaks in A.
