Fig. 3.

Binding of p62 to PVs is independent of GBPs. (A and B) IFNγ-activated WT and coisogenic GBP^chr3−/− MEFs were infected with (A) GFP⁺ C. trachomatis or (B) GFP⁺ T. gondii Pru and stained with anti-p62 (red) and Hoechst (blue). Localization of endogenous p62 to inclusions at 20 hpi or to T. gondii PVs at 1 hpi was quantified. Data are representative of three independent experiments (mean ± SD; n = 3; n.s., not significant). (Scale bar, 10 μm.)