Fig. S1.

p62 colocalizes with ubiquitin-decorated T. gondii PVs in IFNγ-primed BMMs. (A) Unprimed and IFNγ-primed C57BL/6J-derived BMMs were infected with GFP⁺ T. gondii Pru. Colocalization of ubiquitin (red) and p62 (magenta) with T. gondii PVs was monitored at 1 hpi. ***P < 0.0005 (two-tailed unpaired t test). (Scale bar, 10 µm.) (B) Randomly selected ubiquitin- or p62-positive PVs were assessed for costaining with the second marker p62 or ubiquitin, respectively. At least 100 PVs were counted for each condition. Data are representative of three independent experiments (mean ± SD; n = 3).