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Screening of E3-ubiquitin ligases. WT MEFs were transduced with lentiviral vectors encoding shRNAs against indicated genes or an ORF encoding red fluorescent protein (as negative control). Cells were infected with C. trachomatis and treated with IFNγ. Localization of ubiquitin (Ub) to inclusions was monitored at 20 phi. ****P < 0.0001; n.s., not significant (one-way ANOVA). Data are representative of three independent experiments (mean ± SD; n = 3).
