Fig. S5.

p62 promotes TRIM21 recruitment to PVs. (A) WT p62^+/+ and p62^−/− MEFs were infected with GFP⁺ C. trachomatis and treated with IFNγ (100 U/mL) at 3 hpi. Cells were fixed at 20 hpi and stained with anti-TRIM21 (red) and Hoechst (blue). Localization of endogenous TRIM21 to inclusions was quantified. (B) IFNγ-primed (200 U/mL, overnight) p62^+/+ and p62^−/− MEFs were infected with GFP⁺ T. gondii Pru. Localization of endogenous TRIM21 to T. gondii PVs was monitored at 1 hpi. *P < 0.05 (two-tailed unpaired t test). Data are representative of two independent experiments (mean ± SD; n = 3). (Scale bar, 10 μm.)