Fig. 3.

Middle domain of V2 is required for mitochondrial Bak import and tBid-induced cyto c release. (A) HA Western blot (Upper) for the V2^−/− cells transfected with HA-tagged V2 or Chi1 and Bak Western blot (Lower) for the cells expressing V2 or Chi1–Chi4. (B) Cyto c Western blot of the V2^−/− cells transfected with V2 or Chi1–Chi4. (C) HA and Bak Western blot of the V2^−/− cells expressing V2, Chi5, or Chi6. V2^−/− expressing nontagged Chi1 was used as a negative control in an HA Western blot. Western blot (D) and quantification (E) of cyto c release in V2^−/− cells expressing V2, Chi5, or Chi6 are shown.