Table S1.
Strains used in this study
| Strain | mCherry* | GFP | Encoded UL36 product† | Titer, PFU/mL | Source |
| PRV-GS999 | — | — | WT | 3.0 × 109 | Smith and Enquist (41) |
| PRV-GS4957 | — | — | K442R | 1.0 × 109 | Current study |
| PRV-GS5951 | — | — | K442 (GS4957 repair) | 3.0 × 109 | Current study |
| PRV-GS5317 | — | — | K1613R | 6.3 × 108 | Current study |
| PRV-GS5531 | — | — | K1613 (GS5317 repair) | 1.6 × 109 | Current study |
| PRV-GS5380 | — | — | K963 | 2.7 × 109 | Current study |
| PRV-GS5381 | — | — | K1299 | 1.4 × 109 | Current study |
| PRV-GS4284 | pUL25 | — | WT | 2.2 × 109 | Bohannon et al. (43) |
| PRV-GS4747 | pUL25 | — | C26A | 2.1 × 108 | Current study |
| PRV-GS5971 | pUL25 | — | C26 (GS4747 repair) | 2.5 × 109 | Current study |
| PRV-GS5112 | pUL25 | — | K442R | 1.1 × 109 | Current study |
| PRV-GS5933 | pUL25 | — | K442 (GS5112 repair) | 2.7 × 109 | Current study |
| PRV-GS5270 | pUL25 | — | K1613R | 3.0 × 108 | Current study |
| PRV-GS5507 | pUL25 | GFP-pUL36 | WT | 1.5 × 109 | Current study |
| PRV-GS5508 | pUL25 | GFP-pUL36 | C26A (∆DUB) | 3.5 × 108 | Current study |
| PRV-GS5555 | — | CMV > GFP‡ | WT | 1.4 × 108 | Current study |
| HSV1-GS4553 | pUL25 | — | WT | 4.8 × 108 | Current study |
| HSV1-GS5553 | pUL25 | — | C40A (∆DUB)§ | 4.5 × 108 | Current study |
*
mCherry was fused in-frame between pUL25 amino acids 42–43 for PRV and 50–51 for HSV-1.
†
Amino acid numbers are based on PRV strain Becker (GenBank JF797219) and HSV-1 strain F (GenBank GU734771 and KM222724).
‡
Soluble GFP cassette driven by a CMV promoter was inserted into the US4 gene. This virus is also deleted for the UL25 gene.
§
The catalytic cysteine at position 40 has been previously referred to as C65 based on an upstream in-frame ATG being assigned amino acid position 1.