Fig. 6.

Remodeling of live mammalian cell surfaces by site-specific preparation of synthetic mucin-membrane protein chimeras. HEK 293T cells were incubated with norbornene-AA 6 (A and C) or control Boc-AA 7 (B), transfected with plasmids PylT and pMmPylRS-EGFR(128TAG)-GFP (A and B) or with no added plasmid DNA (C), and then treated with synthetic mucin 5 (A–C) [5 µM CF647-poly(α-GalNAc-Ser)₃₀₀-Tz, 4 h]. (Scale bar, 10 µM.)