Figure 1.

Wogonin reduced the incidence and development of CAC. C57BL/6 mice were subjected to an AOM-based CAC induction protocol using three cycles of 2.5% DSS in drinking water. (a) Diagram shows the experimental course of AOM/DSS mouse model. (b) Kaplan–Meier survival curves show the effect of wogonin on the survival of AOM/DSS-treated mice. (c) Body weights of the AOM/DSS group and AOM/DSS+wogonin group were measured. (d) Tumor numbers were counted on day 106. Data represent average tumor numbers±S.D. (n=6). **P<0.01 compared with AOM/DSS group. (e) Tumor sizes were determined using Spot software for microscopic tumors or a caliper for macroscopic tumors. Average tumor size±S.D. is shown; *P<0.05, **P<0.01 compared with AOM/DSS group. (f) Average tumor load was determined. Results are averages±S.D. (n=6). **P<0.01 compared with AOM/DSS group. (g) Histogram showing the size distribution of tumors. (h) Representative images of colons removed from mice in AOM/DSS group and AOM/DSS+wogonin group at day 105 and data statistics of the colon length. (i) H&E stains of serial sections of colons. The arrows showed the possible presence of adenocarcinoma tissues