Figure 1.

Expression of selected mRNAs coding for HDACs in the spinal cord of ALS mice. The cDNAs obtained from total RNA extracted from spinal cord of G93A-SOD1 (dark bars) and G86R-SOD1 (light bars) transgenic mice and their nontransgenic littermates were analyzed by quantitative RT-PCR to assess mRNA expression of HDAC5, HDAC11,SIRT1 and SIRT2. Analysis was performed at different stages of disease starting from early presymptomatic to end stage. The mRNA levels from control mice are set as 1. Results were obtained from at least three different mice from each stage and are expressed as the ratio between the average of values, normalized to the average values of two different housekeeping genes, from nontransgenic and transgenic mice. *P<0.05 with respect to nontransgenic mice of the same age; ^#P<0.05 with respect to presymptomatic or early symptomatic transgenic mice for G93A-SOD1 genotype, and with respect to presymptomatic or symptomatic transgenic mice for G86R-SOD1 genotype