Figure 2.

HDAC protein expression in spinal cord of ALS mice. (a) Western blot analysis of 30 μg of total protein extract from spinal cord of transgenic (+) and nontransgenic (−) G93A-SOD1 mice from symptomatic (123d) to end stage (155d) of disease using antibodies against HDAC5 and HDAC11, β-actin as a loading control and SOD1 to confirm genotypes. hSOD1 indicates exogenous human SOD1, and mSOD1 is endogenous mouse SOD1. (b and c) Same as (a) but starting from early symptomatic stage of disease (113d) using antibodies against SIRT1 and SIRT2. Total brain protein extract was used to confirm tissue specificity. (d) Densitometric analysis of data from n=4 G93A-SOD1 and n=4 nontransgenic mice from different experiments. *P<0.05 and **P<0.01 with respect to nontransgenic mice of the same age; ^##P<0.01 with respect to symptomatic transgenic mice. (e) Western blot analysis of cytosolic (cyt) and nuclear (nuc) protein extract from late symptomatic (145 days) G93A-SOD1 (G93A) and nontransgenic (−) mice using antibodies against SIRT1 and SIRT2. Fractions were controlled for the presence of the nuclear marker lamin B and the cytosolic marker SOD1