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. 2014 Jun 19;5(6):e1296. doi: 10.1038/cddis.2014.247

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Protein expression patterns of SIRT1 and SIRT2 in mouse myoblast C2C12 cells. Untransfected C2C12 (Ctrl) and C2C12 cells constitutively expressing G93A-SOD1 (G93A) were subjected to differentiation protocol to induce expression of the transgene under the myosin heavy chain promoter. (a) Western blot analysis of 20 μg protein from cells lysates obtained 5 days after differentiation of C2C12 cells expressing G93A-SOD1 (G93A) or untransfected (Ctrl) using antibodies against SIRT1 and SIRT2. SOD1 was used to control genotype, β-actin as loading control. (b) Densitometric analysis of n=3 experiments as in (a). Values significantly different from relative controls are indicated with **P<0.01; n.s. indicates values that do not differ significantly