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. 2014 Jun 12;5(6):e1292. doi: 10.1038/cddis.2014.255

Figure 6.

Figure 6

β-Lap attenuates cisplatin-induced decrease in SIRT1 expression through deacetylation of p53. (a–d) Levels of miR-34a expression were analyzed by quantitative RT-PCR in the cochlear tissue (a and b) and HEI-OC1 cells (c and d). (e) HEI-OC1 cells were transfected with pre-miR-34a and control miR for 36 h. Level of SIRT1 protein was analyzed by western blotting. (f–h) Acetylated p53 and total p53 levels were determined by western blotting using anti-acetylated p53 and anti-p53 antibodies in the HEI-OC1 cells and cochlear tissues. *, #P<0.05 by one-way ANOVA compared with the control (*) and cisplatin only group (#). (i and j) Cells were transfected with 100 nM control or p53-specific siRNAs, and then incubated for 24 h. The cells were further treated with cisplatin (20 μM) for 24 h. *, #P<0.05 by one-way ANOVA compared with the control (*) and control-siRNA transfected cells (#), n=3