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Expression of membrane IGF-1R in leukemic cell lines. (a) Flow cytrometric analysis of leukemic cell lines labeled with IGF-1R-phycoerythrin (PE; gray) compared with cells labeled with IgG-PE control antibody (white). (b) Detection of phosphorylated IGF-1R using immunoblotting after IGF-1 ligand stimulation (50 ng/ml). (c) Cell lines were treated with the indicated concentrations of NVP-AEW541 and cell viability was measured using a MTT assay
