Figure 2.

IGFBP7 inhibits the growth of AML cells. (a) Average expression of IGFBP7 measured using qRT-PCR, error bars show the S.D. of a duplicate. (b) Lentivirally transduced leukemic cell lines were analysed for the expression of IGFBP7 by immunoblotting of the lysates (upper panel) and the medium (middle panel). The expression of actin was used as a control for equal loading (lower panel). * indicates an aspecific background band. (c) Cell viability of Kasumi-1 cells transduced with the control (EF-1) or with the IGFBP7 overexpression vector were cultured under low-serum conditions (1%) for 72 h. Bars and error bars show the cell viability and S.D. of a triplicate. (d) AML cells were treated with various concentrations of rhIGFBP7 in Opti-Mem reduced serum medium with 1% FCS for 72 h. Bars represent the average and error bars represent the viability and S.D. of a triplicate. One-way ANOVA analysis was used with a post hoc Tukey test to calculate the P-value *P≤0.05, **P≤0.01, ***P≤0.001