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. 2003 Jun 15;9(6):1241–1245. doi: 10.3748/wjg.v9.i6.1241

Figure 3.

Figure 3

Apoptosis assay by flow cytometry. SW480 cells were treated with doxorubicin (0.86 μmol•l-1) alone, TRAIL (100 μg•l-1) alone or a combination of them for 24 h. The apoptosis-inducing effect was quantified by PI stain flow cytometry assay. A: There was no hypodiploid DNA peak before G1 phase which was similar to normal cells when treated with TRAIL (100 μg•l-1) or doxorubicin (0.86 μmol•l-1) alone. B: There was an obvious hyperdiploid DNA peak before G1 phase in combination treatment, with apoptotic rate of 76.82% ± 1.93%. It was in accordance with cytotoxicity by MTT assay.