p53 protein level by Western blot assay. They were detected after 24 h of treatment. A, B, C, D were respectively normal control, TRAIL (100 μg•l-1), doxorubicin (0.86 μmol•l-1) and TRAIL (100 μg•l-1) + doxorubicin (0.86 μmol•l-1) groups. There was no difference in p53 protein expression among these four groups (P > 0.05).