Table 1.
Name | Sequences | Position |
F1 | T71-TTTCCGGAGYCCCTCTTG | 22-39 |
R1 | M13R2-ATCTGCCAGAGACAGGA | 806-790 |
F2 | T7-GAGGTACTCAGGGGCATTTA | 690-709 |
R2 | M13R-GGATCCAAAGCAAAAGACA | 1567-1549 |
F3 | T7-AGATTTGGAGTTGCATGGATTAAC | 1430-1453 |
R3 | M13R-GGATCCTGAAACATCCA | 2342-2326 |
F4 | M13R-TTCAACAACAGTTTCTACAG | 2231-2250 |
R4 | T7-AGCTTCCTCCTCTGATCTA | 3098-3080 |
F5 | T7-GGGAGATAAGACAGATTCTACAT | 2861-2883 |
R5 | M13R-AGCTTCCACTCTTAACAAA | 3813-3795 |
F6 | M13R-GAATGCTTGGATTGTCTGG | 3634-3652 |
R6 | T7-GATCCATCCCAGTAATCTGA | 4549-4530 |
F7 | T7-ATCAATTGCATTGGCAAC | 4451-4468 |
R7 | M13R-GGATCCGCATCTAATTTAATC | 5278-5258 |
F8 | M13R-GCTGTGGGAGCTGCAGTTGG | 5124-5143 |
R8 | T7-GATCCATGGGACTCTTTCTA | 6027-6008 |
F9 | T7-GAGGAAATTCAATTCTTG | 5872-5889 |
R9 | M13R-GGATGGAGTTCCAGA | 6764-6750 |
F10 | T7-GGAGATGTYGGTCTTGAT | 6657-6674 |
R10 | M13R-AGAGGGGTCTCCGGGAATTT | polyA |
3'RACE | CAAGAGGGGTCTCCGGGAATTTTTTTTTTTTTTTTTTTT | polyA |
T7 indicates adapter with sequence: TAATACGACTCACTATAGGGAGA,
M13R indicates adapter with sequence: AGCGGATAACAATTTCACACAGGA.