Figure 3.

100 g·L^-1 SDS-PAGE analysis of the fusion protein expressed in BL21(DE3). Lane1: control strain BL21(pET) be-fore induction, Lane2: control strain BL21(pET) after 3 h in-duction with IPTG, Lane3: purified recombinant protein Hsp60, Lane4: sonicated supernatant of BL21(pET-Hsp60) cells after 3 h induction with IPTG, Lane5: BL21(pET-Hsp60) cell periplasm protein after 3 h induction with IPTG, Lane6: BL21 (pET- Hsp60) cells after 5 h induction with IPTG, Lane7: BL21 (pET- Hsp60) cells after 3 h induction with IPTG, Lane8: BL21 (pET-Hsp60) cells before induction, Lane9: molecular weight marker (20,30,43,67,94)×10³