Figure 4.
Verification of alkA gene deletion of 2457T028D mutant by PCR. 1: alkA PCR product (amplified from 2457T05), 2: 028pKm PCR product (amplified from 2457T028D), 3: 028Kkm PCR product (amplified from pMD028pKm), 4: DL2000 Marker.
Verification of alkA gene deletion of 2457T028D mutant by PCR. 1: alkA PCR product (amplified from 2457T05), 2: 028pKm PCR product (amplified from 2457T028D), 3: 028Kkm PCR product (amplified from pMD028pKm), 4: DL2000 Marker.