Figure 3.

Simultaneous loss of p53 aggravates the aberrant nuclear morphological phenotypes of Hinfp-ablated cells. (A) IF microscopy reveals drastic changes in nuclear size and shape of cKO MEFs that are exacerbated in dKO MEFs. Nuclei stained with DAPI show increased appearance of irregularly shaped morphology in cells lacking both p53 and Hinfp. Scale Bar 50µm. (B) The bar graph shows distribution of different nuclear morphologies in cKO and dKO MEFs (n = 200 ). (C) IF microscopy of cKO and dKO MEFs stained with α-Tubulin (red) shows increased presence of bi-nucleated cells (white arrowheads) in cKO MEFs. The dKO MEFs have an enhanced phenotype with higher incidence of irregularly shaped fused nuclei (yellow arrowheads). Scale Bar 50 µm.