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. 2015 Jan 20;14(7):1070–1081. doi: 10.1080/15384101.2015.1007781

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© 2015 The Author(s). Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 1. — Stathmin-depleted cells only die if they are delayed in entering mitosis. Hela cells were synchronized with a double thymidine block and transfected with non-targeting siRNA (NT siRNA) or stathmin (STMN) siRNA, and released into medium containing DMSO or the Wee 1 inhibitor MK1775. (A) Western blot of stathmin knockdown, reprobed for tubulin as a loading control. Stathmin level was reduced by approximately 75% compared to non-targeting siRNA transfected cells as shown previously.^11–13 (B) Mitotic index was determined at 2 hour intervals for 12 hours following release from the double thymidine block. Wee1 inhibition restored mitotic entry timing in stathmin-depleted cells to that of control treated cells. (C) Viability was assayed at 48 hours after the second thymidine release via trypan blue exclusion. Relieving the mitotic entry delay in stathmin-depleted cells restored viability to that of control treated cells. ** denotes P < 0.01.