Figure 3.

Cell death occurs asynchronously and over several days for cells depleted of stathmin or pulsed with AURKA and PLK1 inhibitors to delay mitotic entry. Time is plotted from the completion of the last mitosis to the initiation of cell death as detected by morphological changes observed from long term live cell recordings. Data shown are for Hela cells treated with (A) stathmin depletion or (B) synchronized and pulsed with a combination of 300 nM S1451 and 0.8 nM BI2536. Each horizontal bar represents an individual cell. Cells dying at a time of zero reflect cells that died during mitosis.