Fig. 6.

Protamine–RNA-stimulated DCs can induce antigen-specific T cell responses. HLA-A0201⁺ DCs were pulsed with gp100_280–288 short peptide or irrelevant peptide, stimulated with protamine–RNA complexes (pR) formed in 0, 25, or 50 mM NaCl, and co-cultured overnight with Jurkat cells expressing the TCR-v-beta14 receptor specific for the gp100_280–288 peptide in the context of HLA-A0201. The upregulation of CD69 on Jurkat cells was assayed with flow cytometry. The mean percentage of CD69 expression ± SEM on Jurkat cells co-cultured with 6 CD1c⁺ DC and pDC donors is depicted