Fig. 4.

CMV genome-specific in situ hybridization (ISH). ISH was performed on 16 paraffin-embedded primary GBM samples using a CMV DNA probe. Representative results from one positive patient and one control are show. a Patient 12 staining positive for CMV genome using the CMV DNA probe. b Patient 12 staining using a negative control probe. c, d Magnification of the boxed areas in (a) and (b). e Staining of a positive control using the CMV DNA probe. f Staining of a positive control with a negative control probe. g Patient 2 staining negative for CMV genome using the CMV DNA probe. h Patient 12 staining using a negative control probe. Magnification ×100, c, d ×400