Table 1.
Decomposition Kinetics of DOB as a Function of Position within NCPs
| DOB position (SHL) |
local sequencea |
kdec (×10−3s−1) in NCPb |
t½ (min) in NCPb |
t½ (h) in free DNAc |
|---|---|---|---|---|
| 89 (1.5) | TXA | 1.4 ± 0.2 | 8.5 ± 1.1 | 119 ± 6 |
| 73 (0) | AXC | 0.7 ± 0.04 | 16.8 ± 0.8 | 77 ± 5 |
| 119 (4.5) | TXA | 0.7 ± 0.1 | 16.5 ± 1.9 | 133 ± 6 |
| 89* (1.5) | TXT | 1.6 ± 0.1 | 7.0 ± 0.5 | 179 ± 7 |
a
X = DOB.
b
Rate constants are averages ± SD of three experiments, each consisting of three independent reactions.
c
Data were for a single experiment consisting of three independent reactions.
*
indicates different local sequence for DOB89.