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. Author manuscript; available in PMC: 2016 Sep 2.
Published in final edited form as: J Am Chem Soc. 2015 Aug 20;137(34):11022–11031. doi: 10.1021/jacs.5b05478

Table 2.

Effects of Linker Regions on DOB Reactivity

position of
DOB
(SHL)		 DNA
sequence		 H4 variant t½ (min) in
NCPa
89 (1.5) no linker WT 8.5 ± 1.1
89 (1.5) with linker WT 6.5 ± 1.2
89 (1.5) with linker Lys5,8,12,16,20Arg 44.5 ± 6.7
89 (1.5) with linker Lys5,8,12,16,20Arg/His18Ala 109.8 ± 8.3
73 (dyad) no linker WT 16.8 ± 0.8
73 (dyad) with linker WT 10.7 ± 0.4
a

Rate constants are averages ± SD of at least two experiments, each consisting of three independent reactions.