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Destabilization of the NQO1 P187S structure in solution. Two-dimensional 1H/15N-HSQC spectra of NQO1 and NQO1 P187S are shown in (A) and (C), respectively. The distribution of rotational correlation times (τc in ns) for NQO1 and NQO1 P187S are shown in (B) and (D), respectively. The almost complete absence of well-dispersed signals in the HSQC spectrum and of high τc values are indicative of the increased mobility in NQO1 P187S.
