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. Author manuscript; available in PMC: 2015 Oct 21.
Published in final edited form as: FEBS J. 2014 Sep 19;281(20):4691–4704. doi: 10.1111/febs.12975

Fig. 6.

Fig. 6

Analytical size exclusion chromatography of NQO1 and NQO1 P187S. Analytical size exclusion chromatograms of purified NQO1 (continuous line) and NQO1 P187S (dashed line) obtained with a Superdex 200 (10/300) analytical column are shown. The calculated molecular masses of NQO1 (61.3 kDa) and NQO1 P187S (78.33 kDa) indicate that both proteins exist in dimeric forms. The lower elution volume observed for NQO1 P187S indicates deviation from the globular structure adopted by NQO1, thus supporting our NMR data showing that NQO1 P187S has a more disordered structure than NQO1.