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. 2015 Apr 30;14(13):2142–2148. doi: 10.1080/15384101.2015.1041689

Figure 2.

Figure 2.

Plk1 affects AR signaling in PCa cells. (A) Depletion of Plk1 reduced AR level in PCa cells. C4-2 and 22Rv-1 cells were infected with lentivirus to deplete Plk1 for 36 hours and harvested. (B) BI2536 inhibits AR expression in PCa cells. LNCaP, C4-2 and 22Rv-1 cells were treated with or without 200 nmol/L BI2536 and harvested for Real-Time qPCR to measure the mRNA levels of AR and AR variants. (C) AR activity is inhibited by BI2536 in LNCaP cells. LNCaP cells were cultured in RPMI-1640+ 5% CSS medium for 48hrs, then treated with or without 200 nmol/L BI2536 or 1 nmol/L R1881 for 4 hours, and harvested for Real-Time qPCR to measure the mRNA levels of ERG and PSA.