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. 2015 Apr 30;14(13):2142–2148. doi: 10.1080/15384101.2015.1041689

Figure 4.

Figure 4.

Inhibition of AR reduced Plk1 level in LNCaP and C4–2 cells. (A) Cell proliferation was inhibited by 10 μmol/L MDV3100 treatment. (B) Cells were treated with or without 10 μmol/L MDV3100 to inhibit AR activity or not, then treated with or without 1 μmol/L nocodazole to arrest cells in G2/M phase or randomly growing, and harvested for immunoblotting. (C) Cells were treated with thymidine overnight to arrest at G1 phase, released into nocodazole-containing medium ± 10 μmol/L MDV3100 for 12 hours, and harvested for FACS analysis.